Comparison of Images Processed With XCOSM Algorithms
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Raw |
LLS |
EM |
EM-IP |
| XY Section |
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| XZ Section |
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Sections from a 3D image of histoplasma capsulatum
yeast cells
provided by Dr. William Goldman (Department of Molecular Microbiology,
Washington University School of Medicine, St. Louis). The raw image
was collected with a 100x / 1.35 NA oil-immersion objective lens at a
fluorescent wavelength of 520 nm. Pixel sizes are 0.1 micrometers laterally and
0.15 micrometers axially. The 3D image was processed with three XCOSM
algorithms: LLS, EM, and EM with intensity penalty (EM-IP)
The LLS image was generated using a processing
parameter of 0.01. The EM image is the result after 1000 iterations of the EM
algorithm. The EM-IP image is the result after 1000 iterations of the
regularized EM with intensity penalty using a parameter of 1e-6.
Stereo Pairs of the Images
Comparison of LLS images
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LLS (parameter = 1e-2) |
LLS (parameter = 3e-3) |
| XY Section |
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| XZ Section |
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Composed by:
Chrysanthe Preza and Jose Conchello
©2001
